The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable

Fortessa X20c - Fluorescence Detection Map Fluorochromes (common examples) Detector Name Filter Range Laser Wavelength Ultraviolet (355 nm) BUV395 UV379 379/28 BUV496 UV525 525/50 Violet (405 nm) BV421, Pacific Blue, DAPI V450 450/50 Pacific Orange, BV510 V525 525/50 BV605 V610 610/20 BV650 V670 670/30 BV711 V710 710/50 BV785 V780 780/60 Blue

There is also a sample fine rate knob to the left. The LSR-Fortessa can handle over 10,000 events a second without loss of accuracy. Sample Injection Port (SIP):(Tube support arm) SIP has two positions. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1.

Bd fortessa filters

Avbryta Cell sorter, BD Biosciences, e.g. BD Fortessa. All monoclonal antibodies were obtained from BD PharMingen. After washing, cells were analyzed using LSRII Fortessa™ cell analyzer (BD Biosciences, CA), BCreative, BCW, BCX, BCXGS, BCxx, BD, BD Diesel Performance, BD home, BD Crafthouse Fortessa, Craftmade, Craftmill, CraftMore, Craftparts Direct Store, Fledging Store, Fleece, Fleece Performance, Fleetguard Cummins Filtration, Price search results for find the best price for with our price comparison tool. Prices updates daily! Detekteringsblocket i analysatorn Bd tillströmning.

Depressurize the sheath container by pulling up on the vent valve. 4.

BD Fortessa X20 A Configuration and Fluorochromes Laser Diva Parameter Name Fluorochromes 488 nm Blue B 530/30 BB515, FITC, AF488, CFSE, GFP, TO-PRO-1

A1). b. LSR II Fortessa 4L - USZ High-end BD SORP flow cytometer with 4 lasers and 18 measureable parameters (usually 2 light-scatter signals and 16 fluorescent signals). Available laser lines: 405 nm (violet), 488 nm (blue), 561 nm (yellow/green) and 640 nm (red).

488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor

With more than a century of experience and our global reach, BD leads in patient and healthcare worker safety and the technologies that enable medical research and clinical laboratories. BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 635LP PE-Cy5, 7AAD, mPlum C 610/20 600LP PE-Texas Red, PE-CF594 We set up our configuration such that the parameter names specify the laser, detector and filter, 642 C 675/20, etc. as the defaults and added a couple of common filters for corresponding detectors for folks that like to tighten up filters for some panels, like a 712/20 for A700 or BV711, 426/27 for BV421, etc.

3. Depressurize the sheath container by pulling up on the vent valve.
Rollbeskrivning kommunikationschef

BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3.

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.
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Dec 17, 2018 Fluorescence was collected using a 610/20 nm band‐pass (BP) filter and a 450/ 50 nm BP filter for the AT‐specific staining. BD LSRFortessa.